Ncy exists or not, that the Cul5-Roc2 complex has been evolutionarily conserved suggests that it plays an important role in many organisms.Materials and Methods Cell Culture and TransfectionS2 cells were maintained in Schneider's medium supplemented with 10 FBS and 100 U/ml penicillin and 100 ug/ml streptomycin. Cells were transfected using Effectene according to the manufacturer's instructions (
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