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59Eco: 59-TGACAGGAATTCGCTGATGATCCAGAAAACTC-39. Roc2 39Eco: 59-CAGAGGAATTCACCGGTTTATTTTCCCATGCG-39. Protocol used for isolation of GST fusion proteins was previously described [26]. Constructs were transformed into BL21DE3 bacteria and induced by adding IPTG to 0.4 mM. GST-Roc proteins were purified using Glutathione-Sepharose 4B beads (GE Healthcare). Protein concentration was determined by Coomas

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